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To isolate histamine-forming bacteria, a 0.1 ml aliquot of the sample dilute was spread on histamine-forming bacterium isolation agar (HBI agar) fortified with L-histidine (Niven, Jeffreg & Corlett, 1981).
離析氨基酸形成菌，將0.1 ml整除數的稀釋樣品塗佈在以L-組氨酸加強的氨基酸形成菌離析瓊脂(HBI瓊脂) (Niven， Jeffreg， & corlett 1981年)。

Following incubation of the differential agar plates for 4 d at 35 C, colonies with blue or purple colour on the plates were picked and further streaked onto trypticase soy agar (TSA) (Difco) to obtain pure cultures.
在不同培養皿以攝氏35度培養四天之後，摘取在培養皿上藍色或紫色的菌落並進一步在胰酶解酪蛋白大豆瓊脂(TSA) (Difco)上劃下條紋以得到純培養基。
 
Their ability to produce biogenic amines was determined by inoculating the isolates in trypticase soy broth (TSB) (Difco) supplemented with 1% L-histidine (TSBH) and incubated without shaking at 35 C for 24 h. Two millilitres of the culture broth were taken for quantitation of biogenic amines. 
他們生產生物胺的能力是取決於離析菌注入經1% L-組氨酸 (TSBH)補充養分的胰酶解酪蛋白大豆湯(TSB)(Difco)並以攝氏35度不震動方式培養24小時而成。取二毫升培養湯作為生物胺的定量。 

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